云南疣粒野生稻抗白叶枯病多菌系鉴定分析

doi:10.3969/j.issn.1006-8082.2020.03.009

摘要/Abstract

摘要： 水稻白叶枯病是主要的稻作病害之一。相关研究表明，疣粒野生稻高抗白叶枯病，甚至对白叶枯病免疫。为了探索病原菌发生变异情况下，疣粒野生稻抗性的变化情况，本研究利用收集的24个国内外白叶枯病菌系，采用孕穗期剪叶接种的方法，鉴定并评价了36份云南疣粒野生稻的抗病性。结果表明，参试的大部分材料表现为典型的白叶枯病症状，病斑为褐色，病斑长度0.00~0.30 cm；供试材料整体抗性水平较好，44.44%的材料对24个菌株抗性达到了高抗甚至免疫水平，其中，OM2-1在抗性和抗谱方面均表现最优；从居群来说，除景洪景讷和勐海勐往2个居群外，其余居群的抗性达到高抗甚至免疫的程度，耿马孟定的野生稻抗性表现最优；另外，大部分参试的疣粒野生稻杂合度高，同一居群的不同材料对白叶枯病的抗性差异大。

关键词: 白叶枯病, 疣粒野生稻, 抗病性, 鉴定评价

Abstract: Bacterial blight is one of the major rice diseases. Relevant studies showed that Oryza meyeriana has high resistance and even immune to bacterial blight. In order to explore the resistance of Oryza meyeriana on the different pathogens, 36 representative materials were evaluated by inoculating with 24 bacterial blight bacteria lines collected from China and abroad. The results showed that most of the tested materials showed typical symptoms of bacterial blight, and the lesions were brown spots with a lesion length of 0.00~0.30 cm. The overall resistance level of the tested materials was good, 44.44% of the materials reached high resistance and even immune level to 24 strains. Among them, OM2-1 is the best in both resistance and resistance spectrum. In terms of population, the resistance phenotype of other populations except Jinghong Jingne and Menghai Mengwang reached high resistance and even immunity, especially the population of Gengma Mengding had the best resistance. Furthermore, most of the tested materials had high heterozygosity, and the resistance of different materials in the same population to bacterial blight varied greatly.

Key words: bacterial blight, oryza meyeriana, disease resistance, identification of evaluation

中图分类号:

邢佳鑫1,陈玲2,柯学2,钟巧芳2,张敦宇2,付坚2,陈越2,肖素勤2,王玲仙2,王波2,殷富有2,陈丽娟1,程在全2. 云南疣粒野生稻抗白叶枯病多菌系鉴定分析[J]. 中国稻米, DOI: 10.3969/j.issn.1006-8082.2020.03.009 .

参考文献

[1] 夏春，陈红旗，朱旭东. 水稻白叶枯病抗性基因的鉴定、定位、克隆与育种应用[J]. 分子植物育种，2012，10（6）：761-771. [2] 董玉琛. 保护作物多样性发掘有用新基因——试论我国作物种质资源研究当前的任务[J]. 植物遗传资源科学，2000，1（1）：1-3. [3] KHUSH G S, BACLANGCO E, OGAWA T. A new gene for resistance to bacterial blight from O. longistaminata [J]. Rice Genet Newsl, 1990, 12: 9-153. [4] ZHANG Q, WANG C L, ZHAO K J, et al. Development of near–isogenic line CBB23 with a new resistance gene to bacterial blight in rice and its application [J]. Chinese J Rice Sci, 2002, 16（3）: 206-210. [5] AMANTE-BORDEOS A, SITCH L A, NELSON R, et al. Transfer of bacterial blight and blast resistance from the tetraploid wild rice Oryza minuta to cultivated rice, Oryza sativa[J]. Theoretical and Applied Genetics, 1992, 84（3-4）: 345-354 [6] TAN G X, REN X, WENG Q M, et al. Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis [J]. Acta Genetica Sinica, 2004, 31（7）: 724-729. [7] 王春连，赵炳宇，章琦，等. 水稻白叶枯病新抗源Y238的鉴定及其近等基因系培育[J]. 植物遗传资源学报，2004，5（1）：26-30. [8] 郑崇珂，王春连，于元杰，等. 水稻抗白叶枯病新基因Xa32（t）的鉴定和初步定位[J]. 作物学报，2009，35（7）：1 173-1 180. [9] RUAN H H, YAN C Q, AN D R, et al. Identifying and mapping new gene xa32（t） for resistance to bacterial blight （Xanthomonas oryzae pv. oryzae, Xoo） from Oryza meyeriana L [J]. Acta Agricultruae Boreali-occidentalis Sinica, 2008, 17（6）: 170-174. [10] 郭嗣斌，张端品，林兴华. 小粒野生稻抗白叶枯病新基因的鉴定与初步定位[J]. 中国农业科学，2010，43（13）：2 611-2 618. [11] 彭绍裘，魏子生，毛昌祥，等. 云南省疣粒野生稻、药用野生稻和普通野生稻多抗性鉴定[J]. 植物病理学报，1982，12（4）：58-60. [12] 章琦，王春莲，施爱农，等. 野生稻抗稻白叶枯病性（Xanthomonas oryzae pv. oryzae）的评价[J]. 中国农业科学，1994，27（5）：1-9. [13] 程在全，晏慧君，耿显胜，等. 云南疣粒野生稻抗白叶枯病鉴定及叶片组织学观察[J]. 植物病理学报，2008，38（6）：582-291. [14] PETERS S, DAMMEYET B, SCHULZ B. Endophyte-host interactions. I. plant defense reactions to endophytic and pathogenic fungi[J]. Symbiosis, 1998, 25（1）: 193-211. [15] ATTITALLA H I, LATIFFAH Z, SALLEH B, et al. Biology and partial sequencing of an endophytic fusarium oxysporum and plant defense complex[J]. J Mol Evol, 2004,1（2）: 121-144. [16] 余腾琼，肖素勤，殷富有，等. 云南野生稻和地方稻资源抗白叶枯病分析[J]. 植物保护学报，2016，43（5）：774-781.